英文名稱(chēng)：Recombinant Human Brain-Derived Neurotrophic Factor
中文簡(jiǎn)寫(xiě)：【BDNF】重組人腦源性神經(jīng)營(yíng)養(yǎng)因子
Recombinant Human Brain-Derived Neurotrophic Factor 【BDNF】重組人腦源性神經(jīng)營(yíng)養(yǎng)因子 細(xì)胞因子描述：
腦源性神經(jīng)營(yíng)養(yǎng)因子（BDNF）最初從豬腦純化小分子堿性蛋白質(zhì)，是神經(jīng)營(yíng)養(yǎng)因子家族一個(gè)成員，為一種小堿性蛋白質(zhì)。與神經(jīng)生長(zhǎng)因子不同，腦源性神經(jīng)營(yíng)養(yǎng)因子主要在中樞神經(jīng)系統(tǒng)，維持神經(jīng)脊和外胚層基板感覺(jué)神經(jīng)元，這些神經(jīng)元對(duì)神經(jīng)生長(zhǎng)因子無(wú)反應(yīng)。腦源性神經(jīng)營(yíng)養(yǎng)因子在神經(jīng)元存活、分化及突觸可塑性調(diào)節(jié)中起重要作用，參與了大腦學(xué)習(xí)記憶過(guò)程。BDNF生物學(xué)作用主要通過(guò)其受體TrkB介導(dǎo)。
人腦源性神經(jīng)營(yíng)養(yǎng)因子重組蛋白是在大腸桿菌中重組表達(dá)的，是由兩條非糖基化多肽鏈組成的同源二聚體，包含有2 x 119個(gè)氨基酸，總分子量為26，984Da。該人腦源性神經(jīng)營(yíng)養(yǎng)因子重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來(lái)源宿主-大腸桿菌中重組表達(dá)
Recombinant Human Brain-Derived Neurotrophic Factor 【BDNF】重組人腦源性神經(jīng)營(yíng)養(yǎng)因子 細(xì)胞因子實(shí)驗(yàn)須知：
溶解：提供的人腦源性神經(jīng)營(yíng)養(yǎng)因子(BDNF)產(chǎn)品為過(guò)濾滅菌的白色凍干粉末；該凍干粉末是從含人腦源性神經(jīng)營(yíng)養(yǎng)因子(BDNF)的20mM 檸檬酸鈉緩沖液（pH 5）中經(jīng)透析并凍干產(chǎn)生的。我們建議在無(wú)菌的18MΩ-cm水中溶解，使其濃度不低于100ug/ml，后繼實(shí)驗(yàn)過(guò)程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋。
保存：人腦源性神經(jīng)營(yíng)養(yǎng)因子(BDNF)凍干粉末在室溫可保存3周，或置于-18℃凍存用于長(zhǎng)期保存。重新溶解后可以在4℃冷藏保存至少2-7天以上；如果想延長(zhǎng)保存時(shí)間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請(qǐng)避免反復(fù)凍融。
Recombinant Human Brain-Derived Neurotrophic Factor 【BDNF】重組人腦源性神經(jīng)營(yíng)養(yǎng)因子 細(xì)胞因子英文描述：
Synonyms
Brain-Derived Neurotrophic Factor, BDNF, MGC34632.
Introduction
BDNF promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. BDNF is a major regulator of synaptic transmission and plasticity at adult synapses in many regions of the cns. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (ltp), long-term depression (ltd), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
Description
Brain-Derived Neurotrophic Factor Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 2 x 119 amino acids and having a total molecular mass of 26,984 Dalton.
BDNF Human Recombinant is purified by proprietary chromatographic techniques.
Source
Escherichia Coli.
Physical Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
The protein was lyophilized WITHOUT ANY ADDITIVES.
Solubility
It is recommended to reconstitute the lyophilized BDNF in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability
Lyophilized BDNF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BDNF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity
BDNF is greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Amino acid sequence
The sequence of the first five N-terminal amino acids of BDNF was determined and found to be Met-His-Ser-Asp-Pro.
Biological Activity
The ED50, calculated by the dose-dependent induction of ACHE (acetylcholine esterase) in rat basal forebrain primary septal culture is 50 ng/ml corresponding to a Specific Activity of 20,000IU/mg.
Protein content
BDNF quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a standard solution of Brain-derived Neurotrophic Factor as a Reference Standard.
