英文名稱：Recombinant Human Tumor Necrosis Factor-beta
中文簡(jiǎn)稱：【TNF b】重組人腫瘤壞死因子β
Recombinant Human Tumor Necrosis Factor-beta 【TNF b】重組人腫瘤壞死因子β 細(xì)胞因子產(chǎn)品描述：
淋巴毒素alpha,腫瘤壞死因子家族的一員,是一個(gè)淋巴細(xì)胞產(chǎn)生的細(xì)胞因子。LTA高度誘導(dǎo)、分泌和homotrimeric分子存在。LTA形式與lymphotoxin-beta heterotrimers錨lymphotoxin-alpha到細(xì)胞表面。LTA介導(dǎo)多種炎癥、免疫刺激性和抗病毒反應(yīng)。本公司也在開(kāi)發(fā)過(guò)程中參與二級(jí)淋巴器官的形成,在細(xì)胞凋亡過(guò)程中發(fā)揮作用。
人腫瘤壞死因子β重組蛋白是在大腸桿菌中重組表達(dá)的，是一條單鏈、非糖基化的多肽鏈，包含有172個(gè)氨基酸，分子量為18645Da。該人腫瘤壞死因子β重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來(lái)源宿主---大腸桿菌中重組表達(dá)
Recombinant Human Tumor Necrosis Factor-beta 【TNF b】重組人腫瘤壞死因子β 細(xì)胞因子產(chǎn)品使用須知：
溶解：提供的人腫瘤壞死因子β(TNF b)產(chǎn)品為過(guò)濾滅菌的白色凍干粉末；該凍干粉末是從含人腫瘤壞死因子β(TNF b)的溶液（不含添加劑）中凍干產(chǎn)生的。我們建議加無(wú)菌的18MΩ-cm水以配制工作儲(chǔ)存液到不低于100ug/ml，后繼實(shí)驗(yàn)過(guò)程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋。
保存：人腫瘤壞死因子β(TNF b)在室溫可保存3周，或置于-18℃凍存用于長(zhǎng)期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長(zhǎng)保存時(shí)間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請(qǐng)避免反復(fù)凍融。
Recombinant Human Tumor Necrosis Factor-beta 【TNF b】重組人腫瘤壞死因子β 細(xì)胞因子產(chǎn)品英文描述：
Synonyms
Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, LTA, LT, TNFB, TNFSF1.
Introduction
Lymphotoxin alpha, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. LTA is highly inducible, secreted, and exists as homotrimeric molecule. LTA forms heterotrimers with lymphotoxin-beta which anchors lymphotoxin-alpha to the cell surface. LTA mediates a large variety of inflammatory, immunostimulatory, and antiviral responses. LTA is also involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis.
Description
Tumor Necrosis Factor-b Human Recombinant (Lymphotoxin) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 172 amino acids and having a molecular mass of 18645 Dalton.
The TNF-b is purified by standard chromatographic techniques.
Source （來(lái)源）
Escherichia Coli.
Physical Appearance （外觀）
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized protein with no additives.
Solubility （溶解性）
It is recommended to reconstitute the lyophilized Tumor Necrosis Factor-beta in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability （穩(wěn)定性）
Lyophilized Tumor Necrosis Factor-b although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TNF-b should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity （純度）
Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAG.
Amino acid sequence （氨基酸序列）
MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL.
Biological Activity （生物活性）
The ED50 as determined by the cytolysis of murine L929 cells in the presence of Actinomycin D is < 0.05ng/ml, corresponding to a Specific Activity of 20,000,000IU/mg.
Protein （蛋白質(zhì)含量）
content Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.082 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of TNF-b as a Reference Standard.
Usage Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
