產(chǎn)品英文名稱：ITS Liquid Media Supplement (100×)
產(chǎn)品中文名稱：細(xì)胞培養(yǎng)添加因子混合物（合劑）
Sigma 細(xì)胞培養(yǎng)添加因子混合物（合劑）ITS Liquid Media Supplement (100×) I3146-5mL產(chǎn)品英文描述：
Quality Level GMP
sterility sterile-filtered
product line BioReagent
form liquid
impurities endotoxin, tested
suitability suitable for cell culture
storage temp. 2～8°C
Components
Contains 1.0 mg/ml bovine insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite.
Other Notes
ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.
Preparation Note
Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.
Application
Serum-free and reduced serum formulations often require purified forms of the factors normally supplied by serum that are suitable for in vitro growth and differentiation studies. These important factors include insulin, transferrin, selenium, pyruvate, and ethanolamine.
Nutritional studies indicate that the supplement components described are utilized by most mammalian cells. They enhance cell proliferation and decrease the serum requirement of many cell types. When the provided supplement is used with 2 to 4 percent serum, proliferation is reported to be similar to medium supplemented with 10 percent serum.
Sigma 細(xì)胞培養(yǎng)添加因子混合物（合劑）ITS Liquid Media Supplement (100×) I3146-5mL應(yīng)用：
無(wú)血清和降低血清制劑通常需要的是適合于在體外生長(zhǎng)和分化的研究通常由血清供給的因素純化形式。這些重要因素，包括胰島素，轉(zhuǎn)鐵蛋白，硒，丙酮酸鹽和乙醇胺。
Sigma 細(xì)胞培養(yǎng)添加因子混合物（合劑）ITS Liquid Media Supplement (100×) I3146-5mL使用注意事項(xiàng)：
ITS是重組人胰島素，人轉(zhuǎn)鐵蛋白，亞硒酸鈉的混合物。它是一個(gè)普通的細(xì)胞補(bǔ)充設(shè)計(jì)用于在非復(fù)合培養(yǎng)基使用（例如，MEM，RPMI-1640）和復(fù)合培養(yǎng)基（e.g. Ham?F-12，DME/ F-12，MEM）與丙酮酸鈉。
無(wú)酚紅制備Earle氏平衡鹽溶液（EBSS）。
