Fas和Fas配體（FasL）屬于TNF超家族，分別是I型和II型跨膜蛋白。FasL與Fas的結(jié)合觸發(fā)攜帶Fas細(xì)胞的凋亡。凋亡機(jī)制涉及通過(guò)稱為FADD的adaptor分子募集pro-caspase 8蛋白酶，隨后將酶原處理成活性形式。然后這些活性caspases蛋白酶切割各種細(xì)胞底物，導(dǎo)致最終的細(xì)胞死亡。sFasR能夠通過(guò)充當(dāng)FasL誘發(fā)的decoy受體來(lái)抑制FasL誘導(dǎo)的細(xì)胞凋亡（sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL）。
BioGems人類FAS預(yù)包被ELISA（酶聯(lián)免疫吸附測(cè)定）試劑盒是一種固相免疫測(cè)定法，專門(mén)設(shè)計(jì)用于測(cè)量FAS，其帶有FAS特異預(yù)包被鼠單克隆抗體的96孔微孔板。它含有NS0表達(dá)的重組FAS。檢測(cè)抗體是FAS特異的生物素化山羊多克隆抗體。該試劑盒是即用型試劑盒，已經(jīng)過(guò)分析驗(yàn)證。
Human FAS Pre-Coated ELISA試劑盒分析
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Product Details
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Assay Type分析類型:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity靈敏度:
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<3pg/ml
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Range范圍:
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31.2pg/ml-2000pg/ml
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Specificity特異性:
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Natural and recombinant Human FAS
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Uniprot:
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P25445
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Interference干擾:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions保存:
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Store the unopened product at 2 – 8 ℃
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Components組份
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Description
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數(shù)量
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Volume
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Anti-Human FAS Pre-coated 96-well strip microplate
抗人FAS預(yù)包被 96孔微板
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1
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12 strips of 8 wells
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Human FASt Standard?標(biāo)準(zhǔn)樣品
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2
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10 ng vial
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Human FAS Biotinylated antibody (100x)
人FAS生物素化抗體
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1
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130?μL
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Avidin-Biotin-Peroxidase Complex (100x)?
親和素 – 生物素 – 過(guò)氧化物酶復(fù)合物
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1
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130 μL
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Sample Diluent樣品稀釋劑
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1
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30mL
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Antibody Diluent?抗體稀釋劑
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1
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12mL
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Avidin-Biotin-Peroxidase Diluent
親和素 – 生物素 – 過(guò)氧化物酶稀釋劑
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1
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12mL
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Wash Buffer Concentrate (Powder for 1000mL)
洗滌緩沖液濃縮物
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1
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Pack
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Color Developing Reagent
彩色顯影劑
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1
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10mL
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Stop Solution
終止液
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1
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10mL
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Plate Sealers封口機(jī)
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4
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Each
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Reproducibility重復(fù)性
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?
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Intra-Assay
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Inter-Assay
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Sample ID
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1
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2
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3
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1
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2
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3
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n=
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16
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16
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16
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24
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24
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24
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Mean Measured Concentration (pg/mL)
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138
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828
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1507
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146
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839
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1512
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Standard Deviation
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5.8
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25.67
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70.83
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10.95
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45.31
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83.16
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Consistency (%CV)
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4.2
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3.1
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4.7
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7.5
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5.4
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5.5
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Catalog Number : BGK25445
Description
Fas and Fas Ligand (FasL) belong to the TNF superfamily, and are type I and type II transmembrane proteins, respectively. Binding of FasL to Fas triggers apoptosis in Fas-bearing cells. The mechanism of apoptosis involves recruitment of pro-caspase 8 through an adaptor molecule called FADD, followed by processing of the pro-enzyme into active forms. These active caspases then cleave various cellular substrates, leading to the eventual cell death. sFasR is capable of inhibiting FasL-induced apoptosis by acting as a decoy receptor that serves as a sink for FasL.
The BioGems Human FAS Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure FAS with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for FAS. It contains NS0-expressed recombinant FAS. The detection antibody is a biotinylated goat polyclonal antibody specific for FAS. The kit is analytically validated with ready to use reagents.
