Catalog Number :BGK9QYH9
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LIGHT belongs to the TNF family of ligands, and can signal through the herpes virus entry mediator type A receptor (HVEM, TNFRSF14), LTβR, or bind to a decoy receptor, DcR3. It is expressed in splenocytes, activated PBL, CD8+ tumor infiltrating lymphocytes, granulocytes, and monocytes. LIGHT has the ability to active NF-κB, to co-stimulate the activation of lymphocytes and to induce apoptosis in certain human tumor cells.
The BioGems Murine Light Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Light with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for Light. It contains NS0-expressed recombinant Light. The detection antibody is a biotinylated goat polyclonal antibody specific for Light. The kit is analytically validated with ready to use reagents.
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LIGHT屬于TNF配體家族，并且可以通過(guò)皰疹病毒進(jìn)入介質(zhì)A型受體（HVEM，TNFRSF14），LTβR或與decoy受體DcR3結(jié)合而傳導(dǎo)信號(hào)。它在脾細(xì)胞，活化PBL，CD8 +腫瘤浸潤(rùn)淋巴細(xì)胞，粒細(xì)胞和單核細(xì)胞中表達(dá)。LIGHT可以活化NF-κB以共刺激淋巴細(xì)胞的活化并誘導(dǎo)某些人類(lèi)腫瘤細(xì)胞的凋亡。
BioGems鼠Light預(yù)包被ELISA試劑盒是一種固相免疫分析試劑，專(zhuān)門(mén)設(shè)計(jì)用于檢測(cè)Light，并在96孔微孔板上預(yù)包被Light特異性小鼠單克隆抗體。 它含有NS0表達(dá)的重組Light。 檢測(cè)抗體是對(duì)Light特異的生物素化山羊多克隆抗體。該試劑盒經(jīng)過(guò)分析驗(yàn)證，是即用型試劑。
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Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<10pg/ml
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Range:
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93.7pg/ml-6000pg/ml
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Specificity:
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Natural and recombinant Murine Light
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Uniprot:
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Q9QYH9
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 – 8 ℃.
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Components組份
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Description
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Quantity
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Volume
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Anti-Murine Light Pre-coated 96-well strip microplate
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1
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12 strips of 8 wells
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Murine Light Standard
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2
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10 ng vial
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Murine Light Biotinylated antibody (100x)
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1
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130 μL
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Avidin-Biotin-Peroxidase Complex (100x)
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1
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130 μL
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Sample Diluent
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1
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30mL
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Antibody Diluent
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1
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12mL
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Avidin-Biotin-Peroxidase Diluent
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1
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12mL
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Wash Buffer Concentrate (Powder for 1000mL)
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1
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Pack
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Color Developing Reagent
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1
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10mL
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Stop Solution
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1
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10mL
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Plate Sealers
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4
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Each
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Reproducibility可重復(fù)性
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Intra-Assay
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Inter-Assay
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Sample ID
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1
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2
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3
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1
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2
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3
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n=
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16
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16
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16
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24
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24
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24
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Mean Measured Concentration (pg/mL)
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120
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501
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2100
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144.5
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649
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1750
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Standard Deviation
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7.56
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28.55
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102.9
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10.54
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50.62
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96.25
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Consistency (%CV)
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6.3
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5.7
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4.9
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7.3
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7.8
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5.5
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