產(chǎn)品英文名稱：Dkk-1,human
產(chǎn)品中文名稱：人重組Dkk-1蛋白

Dkk-1,human產(chǎn)品介紹：
DKK-1是重組人Dickkopf相關(guān)蛋白1的簡稱，是DKK蛋白家族（DKK1-4）的一員。DKK最初被認(rèn)為是非洲爪蟾蜍的前期成形細(xì)胞。通過機(jī)理研究發(fā)現(xiàn)DKK-1通過與共同受體LRP5/6一起形成抑制復(fù)合物，可以有效抑制Wnt/β-catenin信號通路。研究人員發(fā)現(xiàn)敲除DKK-1基因小鼠的腦部無法形成，從而證實Wnt/β-catenin信號通路的抑制在脊椎動物的后模式與前模式發(fā)展中必不可少。
StemRD公司生產(chǎn)的人重組DKK-1蛋白來源于人的293細(xì)胞，是含有235個氨基酸片段，分子量為3740kDa的糖蛋白。通過諸如HPLC等一系列色譜分析方法對其進(jìn)行純化。

Dkk1,human 產(chǎn)品特性：
» Dkk1,human經(jīng)SDS-PAGE鑒定純度大于90%
» 生物活性在培養(yǎng)人細(xì)胞中用TCF受體基因檢測確定，拮抗劑WNT-3a 100 ng/mL時其IC50在100 - 500 ng/ml之間
» 本產(chǎn)品只限于科學(xué)研究，非人體使用

Dkk-1,human產(chǎn)品英文簡述：
DKK-1, Dickkopf-related protein-1, is a member of the DKK protein family which includes DKK-1 through DDK-4. DKK was originally identified as a head-forming molecule in Xenopus. Mechanistic studies showed that DKK-1 inhibits the Wnt /β-catenin signaling pathway by forming inhibitory complexes with co-receptor LRP5/6. Inhibition of Wnt/β-catenin signaling is essential for posterior patterning and anterior development in vertebrates, supported by the discovery that DKK-1 knockout mice lack head formation.
StemRD expressed recombinant human DKK-1 in human 293 cells as a 37-40 kDa glycoprotein containing 235 amino-acid residues. It is purified by aseries of chromatography including HPLC.

• Purity: Greater than 90% as determined by SDS-PAGE analysis
• Biological Activity: The activity was determined by using a TCF reporter gene assay in cultured human cells. The IC50 ranges from 100 - 500 ng/ml in the inhibition of 100 ng/mL WNT-3a activity. Activity in other assays should be determined by each individual setting.
• For Research Use Only. Not for Use in Human