產(chǎn)品英文名稱：Stemcell 19782 EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit
產(chǎn)品中文名稱：Stemcell 19782 EasySep™ 小鼠CD4+CD25+調(diào)節(jié)性T細(xì)胞分離試劑盒

Stemcell 19782 EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit 產(chǎn)品簡介
Stemcell 19782 EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit 通過陽性選擇從小鼠脾細(xì)胞的單細(xì)胞懸液或其他組織中分離CD4+ CD25+ T細(xì)胞。 CD4+ T細(xì)胞富集使用EasySep™小鼠CD4+ T細(xì)胞預(yù)富集試劑盒的陰性選擇。然后CD25+細(xì)胞從預(yù)富集的細(xì)胞中使用EasySep™小鼠CD25陽性選擇試劑盒。富集所有的CD4+ T細(xì)胞時，不進(jìn)行CD25+細(xì)胞的選擇，我們建議的EasySep™小鼠CD4+ T細(xì)胞富集試劑盒（產(chǎn)品編號19752）。

Stemcell 19782 EasySep™ 小鼠CD4+CD25+調(diào)節(jié)性T細(xì)胞分離試劑盒產(chǎn)品組分
Stemcell 19782 EasySep™ 小鼠CD4+CD25+調(diào)節(jié)性T細(xì)胞分離試劑盒有兩個部分
• EasySep™ Mouse CD4+ T Cell Pre-Enrichment Kit
• EasySep™ Mouse CD25 Positive Selection Kit

Stemcell 19782 EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit 推薦使用
Stemcell 19782 EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit is designed for the isolation of mouse CD4+CD25+ cells from single cell suspensions of splenocytes or other tissues by negative followed by positive selection

Stemcell 19782 EasySep™ 小鼠CD4+CD25+調(diào)節(jié)性T細(xì)胞分離試劑盒所需設(shè)備
• EasySep™ Magnet (Catalog #18000), or
• "The Big Easy" EasySep™ Magnet (Catalog #18001)

Stemcell 19782 EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit 保存方法
EASYSEP® MOUSE CD4+ T CELL PRE-ENRICHMENT COCKTAIL
Product stable at 2 – 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 22°C), and should be refrigerated upon receipt.
EASYSEP® BIOTIN SELECTION COCKTAIL
Product stable at 2 – 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 22°C), and should be refrigerated upon receipt.
EASYSEP® D MAGNETIC PARTICLES
Product stable at 2 – 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 - 22°C), and should be refrigerated upon receipt.
NORMAL RAT SERUM
Product stable at 2 – 8°C until expiry date as indicated on label. Stable for at least 2 years if stored at -20°C. Contents have been sterility tested.
EASYSEP® BLOCKING SOLUTION
Product stable at 2 – 8°C or room temperature (15 - 22°C) until expiry date as indicated on label. Contents have been sterility tested. Please note that repeated exposure to air may cause some crystallization to occur around the edge of the tube. This crystallization does not affect the performance of the blocking solution.

Stemcell 19782 EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit 實驗資料
FACS Profile Results with EasySep® Mouse CD4+CD25+ T Cell Positive Selection Kit

Starting with mouse splenocytes, the CD4+CD25+ cell content of the selected cells typically ranges from 85 - 97%.

Typical EasySep™ Procedure for Mouse Cells


Stemcell 19782 EasySep™ 小鼠CD4+CD25+調(diào)節(jié)性T細(xì)胞分離試劑盒相關(guān)產(chǎn)品訂購信息
Stemcell  19792  EasySep™ Mouse CD4+CD25+ Regulatory T Cell Isolation Kit  Immunomagnetic Negative Followed by Positive Selection Kit 
Stemcell  18001  The Big Easy EasySep™ Magnet  Immunomagnetic Column-Free Magnet
Stemcell  20000  RoboSep™  RoboSep™ - The Fully Automated Cell Separator 
Stemcell  18000  EasySep™ Magnet  Immunomagnetic Column-Free Magnet 
Stemcell  19752  EasySep™ Mouse CD4+ T Cell Enrichment Kit  Immunomagnetic Negative Selection Kit   
For research use only. Not intended for human or animal diagnostic or therapeutic uses.