產(chǎn)品英文名稱：Dkk-1,human DKK-025,5ug
產(chǎn)品中文名稱：人源Dkk-1蛋白WNT信號通路抑制劑,5ug

Dkk-1,human DKK-025,5ug 產(chǎn)品簡介
DKK-1是重組人Dickkopf相關(guān)蛋白1的簡稱，是DKK蛋白家族（DKK1-4）的一員。DKK最初被認(rèn)為是非洲爪蟾蜍的前期成形細(xì)胞。通過機(jī)理 研究發(fā)現(xiàn)DKK-1通過與共同受體LRP5/6一起形成抑制復(fù)合物，可以有效抑制Wnt/β-catenin信號通路。研究人員發(fā)現(xiàn)敲除DKK-1基因小 鼠的腦部無法形成，從而證實Wnt/β-catenin信號通路的抑制在脊椎動物的后模式與前模式發(fā)展中必不可少。
StemRD公司生產(chǎn)的人重組Dkk-1,human DKK-025,5ug蛋白干細(xì)胞生長分化調(diào)整因子來源于人的293細(xì)胞，是含有235個氨基酸片段，分子量為3740kDa的糖蛋白。通過諸如HPLC等一系列色譜分析方法對其進(jìn)行純化。
Wnt 信號轉(zhuǎn)導(dǎo)通路參與了多種生物學(xué)過程的調(diào)控, 包括胚胎的生長和形態(tài)發(fā)育、組織的穩(wěn)定、能量代謝的平衡以及干細(xì)胞的維持。Wnt 通路的失調(diào)與人類重大疾病有密切聯(lián)系。
近年來人們在對干細(xì)胞的研究中發(fā)現(xiàn),Wnt 信號通路對于表皮干細(xì)胞、腸干細(xì)胞、造血干細(xì)胞、神經(jīng)干細(xì)胞、胚胎干細(xì)胞以及腫瘤干細(xì)胞的維持都起著重要的調(diào)控作用。所以, 深入研究Wnt 信號通路的調(diào)控機(jī)理, 尋找藥物治療的靶蛋白,是近年來生物學(xué)和醫(yī)學(xué)研究的熱門領(lǐng)域。

人源Dkk-1蛋白WNT信號通路抑制劑,5ug產(chǎn)品特性
» 人源Dkk-1蛋白干細(xì)胞生長分化調(diào)整因子WNT信號通路抑制劑,5ug鑒定純度大于90%
» 人源Dkk-1蛋白干細(xì)胞生長分化調(diào)整因子WNT信號通路抑制劑,5ug生物活性在培養(yǎng)人細(xì)胞中用TCF受體基因檢測確定，拮抗劑WNT-3a 100 ng/mL時其IC50在100 - 500 ng/ml之間

Dkk-1,human DKK-025,5ug 保存方法
The lyophilized protein is stable for at least one year if stored at -80 degreeC. Reconstituted protein is stable for at least four weeks at 4 degree C, but should be stored in aliquots at -80 degree C for longer term. Avoid
repeated freeze and thaw.

Dkk-1,human DKK-025,5ug 產(chǎn)品英文描述
DKK-1, Dickkopf-related protein-1, is a member of the DKK protein family which includes DKK-1 through DDK-4. DKK was originally identified as a head-forming molecule in Xenopus. Mechanistic studies showed that DKK-1 inhibits the Wnt /β-catenin signaling pathway by forming inhibitory complexes with co-receptor LRP5/6. Inhibition of Wnt/β-catenin signaling is essential for posterior patterning and anterior development in vertebrates, supported by the discovery that DKK-1 knockout mice lack head formation.
StemRD expressed recombinant human Dkk-1,human DKK-025,5ug in human 293 cells as a 37-40 kDa glycoprotein containing 235 amino-acid residues. It is purified by aseries of chromatography including HPLC.
•Dkk-1,human DKK-025,5ug Purity: Greater than 90% as determined by SDS-PAGE analysis
•Dkk-1,human DKK-025,5ug Biological Activity: The activity was determined by using a TCF reporter gene assay in cultured human cells. The IC50 ranges from 100 - 500 ng/ml in the inhibition of 100 ng/mL WNT-3a activity. Activity in other assays should be determined by each individual setting.

人源Dkk-1蛋白WNT信號通路抑制劑系列產(chǎn)品訂購信息
StemRD DKK-025  25ug  優(yōu)惠價格請來電咨詢
StemRD DKK-100  100ug  優(yōu)惠價格請來電咨詢
StemRD DKK-500  500ug  優(yōu)惠價格請來電咨詢
StemRD DKKFC-050  50ug  優(yōu)惠價格請來電咨詢
StemRD DKKFC-250  250ug  優(yōu)惠價格請來電咨詢
StemRD DKKFC-1000  1000ug  優(yōu)惠價格請來電咨詢
For research use only. Not intended for human or animal diagnostic or therapeutic uses.