英文名稱：Recombinant Human Hepatocyte Growth Factor，CHO
中文簡稱：【HGF CHO】重組人肝細胞生長因子,CHO表達

Recombinant Human Hepatocyte Growth Factor，CHO 【HGF CHO】重組人肝細胞生長因子,CHO表達 細胞因子產(chǎn)品描述：

肝細胞生長因子（hepatocyte growth factor，HGF）最初作為一種肝細胞有絲分裂原是從肝部分切除大鼠的血清中分離得到的。HGF是來源于間充質(zhì)的多效生長因子，可以促進多種細胞的分裂和生長。以旁分泌、內(nèi)分泌、自分泌形式在保護器官，延長組織老化方面發(fā)揮作用。HGF首先以無活性的單鏈蛋白（HGF SF）形式分泌，經(jīng)肝細胞生長因子激活因子切割成成熟的異二聚體HGF，機體內(nèi)HGFA可激活HGF SF前體，使之從單鏈前體變成雙鏈活性形式，是調(diào)節(jié)細胞外HGF SF活性的關(guān)鍵步驟。HGF在促進肝細胞再生的同時參與正常組織的損傷后修復(fù)、細胞的運動以及腫瘤的形成、浸潤和轉(zhuǎn)移、分化以及腫瘤血管行程中發(fā)揮重要的作用。
人肝細胞生長因子，CHO表達重組蛋白是在CHO中國倉鼠卵巢細胞中重組表達的，是由兩條非糖基化多肽鏈，即463個氨基酸的α鏈和234個氨基酸的β鏈組成的異源二聚體，總分子量為75kDa。該人肝細胞生長因子，CHO表達重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來源宿主---CHO中國倉鼠卵巢細胞中重組表達

Recombinant Human Hepatocyte Growth Factor，CHO 【HGF CHO】重組人肝細胞生長因子,CHO表達 細胞因子產(chǎn)品使用須知：

溶解：提供的人肝細胞生長因子,CHO表達(HGF CHO)產(chǎn)品為過濾滅菌的白色凍干粉末；該凍干粉末是從含1mg/ml人肝細胞生長因子,CHO表達(HGF CHO)的0.02% Tween 80，磷酸鹽緩沖液（pH 7.4）中凍干產(chǎn)生的。我們建議加無菌的18MΩ-cm水以配制工作儲存液到100ug/ml，后繼實驗過程中可根據(jù)實驗要求進一步稀釋。
保存：人肝細胞生長因子,CHO表達(HGF CHO)凍干粉末在室溫可保存3周，或置于-18℃凍存用于長期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長保存時間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請避免反復(fù)凍融。

Recombinant Human Hepatocyte Growth Factor，CHO 【HGF CHO】重組人肝細胞生長因子,CHO表達 細胞因子產(chǎn)品英文描述：

Synonyms 

Scatter Factor (SF), Hepatopoietin (HPTA), HGF.

Introduction 

Hepatocyte Growth Factor (HGF) is a multifunctional growth factor which regulates both cell growth and cell motility. It exerts a strong mitogenic effect on hepatocytes and primary epithelial cells. HGF synergizes with Interleukin-3and GM-CSFto stimulate colony formation of hematopoietic progenitor cells in vitro and may, therefore, also modulate hematopoiesis.

Description 

Hepatocyte Growth Factor Human Recombinant produced in CHO is a heterodimer, non-glycosylated, polypeptide chain consisting an a-chain of 463 amino acids and b-chain of 234 having a total molecular mass of 75kDa.
The HGF is purified by proprietary chromatographic techniques.

Source  （來源）

Chinese Hamster Ovary Cells.

Physical Appearance  （外表）

Sterile Filtered White lyophilized (freeze-dried) powder.

Formulation 

The protein was lyophilized from a concentrated solution containing Phosphate-Buffered Saline with 0.02% Tween 80, pH 7.4.

Solubility  （溶解性）

It is recommended to reconstitute the lyophilized Hepatocyte Growth Factor in sterile 18M-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

Stability    （穩(wěn)定性）

Lyophilized Hepatocyte Growth Factor although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution HGF should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.

Purity         （純度）

Greater than 97.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Amino acid sequence   （氨基酸序列）

Agrees with the sequence of native human HGF.

Biological Activity    （生物活性）

The ED50, calculated by the dose-dependant proliferation of monkey 4MBr-5 indicator cells was found to be 20-40ng/ml corresponding to a Specific Activity of 25,000-50,000IU/mg.

Protein content     （蛋白質(zhì)含量）

Protein quantitation was carried out by two independent methods:
1. UV spectroscopy at 280 nm using the absorbency value of 1.83 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).
2. Analysis by RP-HPLC, using a calibrated solution of HGF as a Reference Standard.

Usage 

ProSpec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.