英文名稱：ecombinant Human Interleukin-4 CHO
中問(wèn)簡(jiǎn)稱：【IL 4 CHO】重組人白介素-4,CHO表達(dá)
Recombinant Human Interleukin-4 CHO 【IL 4 CHO】重組人白介素-4,CHO表達(dá) 細(xì)胞因子描述：
IL4是多向性的激活T細(xì)胞產(chǎn)生的細(xì)胞因子。IL4是白介素4受體的配體。IL13白介素4受體也綁定,這可能導(dǎo)致許多重疊的細(xì)胞因子和IL13功能。STAT6、傳感器信號(hào)和轉(zhuǎn)錄激活劑,已被證明在介導(dǎo)免疫中發(fā)揮核心作用的監(jiān)管這一細(xì)胞因子的信號(hào)。這種基因,IL3 IL5、IL13 CSF2形成一個(gè)細(xì)胞因子基因簇5號(hào)染色體上q,特別是接近IL13基因。IL4,IL13 IL5發(fā)現(xiàn)監(jiān)管協(xié)調(diào)由幾個(gè)遠(yuǎn)程監(jiān)管元素在染色體上的120多千堿基范圍。兩個(gè)或者拼接成績(jī)單變異的基因編碼已報(bào)告不同的亞型。
人白介素-4，CHO表達(dá)重組蛋白是在CHO中國(guó)倉(cāng)鼠卵巢細(xì)胞中重組表達(dá)的，是一條單鏈、非糖基化的多肽鏈，包含有129個(gè)氨基酸，分子量為14kDa，由于糖基化而導(dǎo)致在SDS-PAGE電泳顯示分子量為18 kDa。該人白介素-4，CHO表達(dá)重組蛋白最終由標(biāo)準(zhǔn)的色譜技術(shù)純化出。
來(lái)源宿主---CHO中國(guó)倉(cāng)鼠卵巢細(xì)胞中重組表達(dá)。
Recombinant Human Interleukin-4 CHO 【IL 4 CHO】重組人白介素-4,CHO表達(dá) 細(xì)胞因子使用須知：
溶解：提供的人白介素-4,CHO表達(dá)(IL 4 CHO)產(chǎn)品為過(guò)濾滅菌的白色凍干粉末；該凍干粉末是從含人白介素-4,CHO表達(dá)(IL 4 CHO)的磷酸鹽緩沖鹽水(pH 7.4)中凍干產(chǎn)生的。我們建議加無(wú)菌的18MΩ-cm水以配制工作儲(chǔ)存液到100ug/ml，后繼實(shí)驗(yàn)過(guò)程中可根據(jù)實(shí)驗(yàn)要求進(jìn)一步稀釋。
保存：人白介素-4,CHO表達(dá)(IL 4 CHO)凍干粉末在室溫可保存3周，或置于-18℃凍存用于長(zhǎng)期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長(zhǎng)保存時(shí)間，建議分裝（加載體蛋白如0.1% HSA或 BSA）后置于-18℃凍存。請(qǐng)避免反復(fù)凍融。
Recombinant Human Interleukin-4 CHO 【IL 4 CHO】重組人白介素-4,CHO表達(dá) 細(xì)胞因子英文描述：
Synonyms
BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra.
Introductionn
IL4 is a pleiotropic cytokine produced by activated T cells. IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, which may contribute to many overlapping functions of this cytokine and IL13. STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. Two alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.
Description
Interleukin-4 Human Recombinant produced in CHO is a single, non-glycosylated polypeptide chain containing 129 amino acids and having a molecular mass of 14 kDa, the glycosilation of IL-4 migrates as 18 kDa on SDS-PAGE.
The IL-4 is purified by proprietary chromatographic techniques.
Source
Chinese Hamster Ovarian Cells.
Physical Appearance （外觀）
Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation
Lyophilized from a Phosphate- Buffered Saline (pH 7.4).
Solubility （溶解性）
It is recommended to reconstitute the lyophilized Interleukin-4 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability （穩(wěn)定性）
Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C.
For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).
Please prevent freeze-thaw cycles.
Purity （純度）
Greater than 97.0% as determined by
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Biological Activity （生物活性）
Measured in a cell proliferation assay using TF-1 cells. The ED50 for this effect is 0.05-0.2ng/ml corresponding to a Specific Activity of 5,000,000-20,000,000IU/mg.
