英文名稱：Recombinant Mouse Macrophage Colony Stimulating Factor
中文簡稱：【mM CSF】重組小鼠巨噬細胞集落刺激因子

 產(chǎn)品鏈接：重組小鼠巨噬細胞集落刺激因子

Recombinant Mouse Macrophage Colony Stimulating Factor 【mM CSF】重組小鼠巨噬細胞集落刺激因子 細胞因子產(chǎn)品描述：

巨噬細胞集落刺激因子(Macrophage colony-stimulating factor，M-CSF)，也稱集落刺激因子-1(CSF-1)，是近年來受到廣泛重視的具有多種生物學功能的細胞因子。M-CSF對單核細胞的增殖、分化及維持其活性有重要作用。另外它還調節(jié)胎盤功能，促進骨吸收。M-CSF為由鏈間二硫鍵連接而成的同源二聚體糖蛋白。廣泛存在于血清、尿及其他組織中。人M-CSF與小鼠M-CSF在其DNA序列上有70%的同源性。M-CSF可以在間質細胞如成纖維細胞、成骨細胞及內皮細胞中合成，也可在激活的巨噬細胞、B細胞、T細胞及多種腫瘤細胞產(chǎn)生。
小鼠巨噬細胞集落刺激因子重組蛋白是在大腸桿菌中重組表達的，是一條由二硫鍵連接兩條非糖基化多肽鏈形成的同源二聚體，包含有2 x 156個氨基酸，總分子量為36.4kDa。該小鼠巨噬細胞集落刺激因子重組蛋白最終由標準的色譜技術純化出。
來源宿主---大腸桿菌中重組表達

Recombinant Mouse Macrophage Colony Stimulating Factor 【mM CSF】重組小鼠巨噬細胞集落刺激因子 細胞因子產(chǎn)品使用須知：
溶解：提供的小鼠巨噬細胞集落刺激因子(mM CSF)產(chǎn)品為過濾滅菌的白色凍干粉末；該凍干粉末是從含1mg/ml小鼠巨噬細胞集落刺激因子(mM CSF)的5mM Tris，pH 8.0 ，100mM NaCl溶液中凍干產(chǎn)生的。我們建議加無菌的18MΩ-cm水以配制工作儲存液到不低于100ug/ml，后繼實驗過程中可根據(jù)實驗要求進一步稀釋。
保存：小鼠巨噬細胞集落刺激因子(mM CSF)在室溫可保存3周，或置于-18℃凍存用于長期保存。重新溶解后可以在4℃冷藏保存至少2-7天；如果想延長保存時間，建議分裝后置于-18℃凍存。請避免反復凍融。

Recombinant Mouse Macrophage Colony Stimulating Factor 【mM CSF】重組小鼠巨噬細胞集落刺激因子 細胞因子產(chǎn)品英文描述：

Synonyms 

CSF-1, Lanimostim, MCSF, M-CSF.

Introduction 

Granulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.

Description 

Macrophage Colony Stimulating Factor Mouse Recombinant produced in E.coli is a disulfide linked homodimer, non-glycosylated, polypeptide chain containing 2 x 156 amino acids and having a total molecular mass of 36.4 KD.
MCSF is purified by proprietary chromatographic techniques.

Source  （來源）

Escherichia Coli.

Physical Appearance  （物理特征）

Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation  The protein was lyophilized with 0.5xPBS, pH 8.0.

Solubility  （穩(wěn)定性）

It is recommended to reconstitute the lyophilized M-CSF in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Stability  Lyophilized M-CSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C.
Please prevent freeze-thaw cycles.

Amino acid sequence  （氨基酸序列）

MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP.

Purity  （純度）

Greater than 98.0% as determined by:
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.

Biological Activity  （生物活性）

The ED50, as calculated by the dose-dependant stimulation of the proliferation of murine M-NFS-60 indicator cells is typically 1.1-1.6ng/ml corresponding to 909,090-625,000 U/mg.