β-NGF is a neurotrophic factor structurally related to BDNF, NT-3 and NT-4. These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. β-NGF is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. β-NGF also acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival.
The BioGems Murine β-NGF Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure β-NGF with a 96-well strip plate that is pre-coated with mouse monoclonal antibody specific for β-NGF. It contains NS0-expressed recombinant β-NGF. The detection antibody is a biotinylated goat polyclonal antibody specific for β-NGF. The kit is analytically validated with ready to use reagents.
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β-NGF是與BDNF，NT-3和NT-4蛋白結(jié)構(gòu)相關(guān)的神經(jīng)營(yíng)養(yǎng)因子。這些蛋白屬于可能與穩(wěn)定二聚體結(jié)構(gòu)相關(guān)的半胱氨酸結(jié)家族生長(zhǎng)因子。 β-NGF是通過(guò)其受體β-NGFR傳導(dǎo)信號(hào)的強(qiáng)效神經(jīng)營(yíng)養(yǎng)因子，并且在感覺(jué)和交感神經(jīng)系統(tǒng)的發(fā)育和保存中起關(guān)鍵作用。 β-NGF還充當(dāng)B淋巴細(xì)胞的生長(zhǎng)和分化因子，并增強(qiáng)B細(xì)胞存活。
BioGems鼠β-NGF預(yù)包被ELISA（酶聯(lián)免疫吸附試驗(yàn)）試劑盒采用固相免疫測(cè)定試劑盒，專(zhuān)門(mén)設(shè)計(jì)用于檢測(cè)β-NGF，其中96孔微孔板預(yù)包被β-NGF特異小鼠單克隆抗體NGF。它含有NS0表達(dá)的重組β-NGF。檢測(cè)抗體是對(duì)β-NGF特異的生物素化山羊多克隆抗體。該試劑盒經(jīng)過(guò)分析驗(yàn)證，是即用型試劑。
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Product Details
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Assay Type:
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Solid Phase Sandwich ELISA: 96-Well Strip Plate
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Sensitivity:
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<1pg/ml
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Range:
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31.2pg/ml-2000pg/ml
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Specificity:
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Natural and recombinant Murine β-NGF
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Uniprot:
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P01139
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Interference:
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No detectable cross-reactivity with other relevant proteins
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Storage Conditions:
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Store the unopened product at 2 – 8 ℃.
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Components組份
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Description
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Quantity
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Volume
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Anti-Murine β-NGF Pre-coated 96-well strip microplate
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1
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12 strips of 8 wells
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Murine β-NGF Standard
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2
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10 ng vial
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Murine β-NGF Biotinylated antibody (100x)
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1
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130 μL
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Avidin-Biotin-Peroxidase Complex (100x)
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1
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130 μL
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Sample Diluent
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1
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30mL
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Antibody Diluent
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1
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12mL
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Avidin-Biotin-Peroxidase Diluent
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1
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12mL
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Wash Buffer Concentrate (Powder for 1000mL)
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1
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Pack
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Color Developing Reagent
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1
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10mL
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Stop Solution
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1
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10mL
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Plate Sealers
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4
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Each
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Reproducibility?可重復(fù)性
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Intra-Assay
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Inter-Assay
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Sample ID
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1
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2
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3
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1
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2
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3
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n=
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16
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16
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16
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24
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24
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24
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Mean Measured Concentration (pg/mL)
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136
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727
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1326
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153
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839
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1645
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Standard Deviation
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5.3
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41.44
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83.54
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6.58
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53.7
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121.73
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Consistency (%CV)
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3.9
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5.7
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6.3
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4.3
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6.4
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7.4
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窗體底端
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