Human Lineage Cocktail 4 (lin 4) (CD2, CD3, CD4, CD7, CD8, CD10, CD11b, CD14, CD19, CD20, CD56, CD235a)
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產(chǎn)品英文名稱:Human Lineage Cocktail 4 (lin 4) (CD2, CD3, CD4, CD7, CD8, CD10, CD11b, CD14, CD19, CD20, CD56, CD235a)
產(chǎn)品中文名稱:人血系混合物4(lin 4)( CD2, CD3, CD4, CD7, CD8, CD10, CD11b, CD14, CD19, CD20, CD56, CD235a)
Human Lineage Cocktail 4 (lin 4) (CD2, CD3, CD4, CD7, CD8, CD10, CD11b, CD14, CD19, CD20, CD56, CD235a)產(chǎn)品描述
FITC標(biāo)記的人血系混合物4設(shè)計用于與來自主要造血譜系的細(xì)胞反應(yīng),比如T淋巴細(xì)胞、B淋巴細(xì)胞、單核細(xì)胞/巨噬細(xì)胞、NK細(xì)胞、紅細(xì)胞和粒細(xì)胞等。這種預(yù)稀釋的混合物由12個FITC標(biāo)記的抗體構(gòu)成,用于排除標(biāo)記血系陽性標(biāo)記細(xì)胞有助于流式細(xì)胞儀鑒定人臍帶血和骨髓廇中的陰性標(biāo)記造血干細(xì)胞。
包含成份:clone RPA-2.10, 識別CD2; HIT3a, 識別CD3; RPA-T4, 識別CD4; M-T701, 識別CD7; HIT8a, 識別CD8; HI10a, 識別CD10; ICRF44, 識別CD11b; M5E2, 識別CD14; SJ25-C1, 識別CD19; 2H7, 識別CD20; B159, 識別CD56和GA-R2(HIR2), 識別CD235a.額外熒光標(biāo)記試劑可與FITC人血系混合物4, 比如CD34, CD38, CD90和CD45RA結(jié)合,以描述來自臍帶血或骨髓廇造血干細(xì)胞群。
人血洗混合物4(lin 4)( CD2, CD3, CD4, CD7, CD8, CD10, CD11b, CD14, CD19, CD20, CD56, CD235a)的保存條件
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.The antibody was conjugated with FITC under optimum conditions, and unreacted FITC was removed.
Human Lineage Cocktail 4 (lin 4) (CD2, CD3, CD4, CD7, CD8, CD10, CD11b, CD14, CD19, CD20, CD56, CD235a)的注意事項
1.This reagent has been pre-diluted for use at the recommended Volume per Test. We typically use 1 × 10^6 cells in a 100-μl experimental
sample (a test).
2. Please refer to www.bdbiosciences.com/pharmingen/protocols for technical protocols.
3.For fluorochrome spectra and suitable instrument settings, please refer to our Multicolor Flow Cytometry web page at www.bdbiosciences.com/colors.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
5.Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before
discarding to avoid accumulation of potentially explosive deposits in plumbing.
6. Brilliant Violet™ 421 is a trademark of Sirigen.
7. Brilliant Violet™ 605 is a trademark of Sirigen.
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