產品英文名稱: EasySep™ RosetteSep™ Complete Kit for CD4+CD25+ T Cells
產品中文名稱: EasySep™ RosetteSep™ CD4+CD25+ T細胞完整試劑盒

EasySep™ RosetteSep™ Complete Kit for CD4+CD25+ T Cells 產品英文描述
The Complete Kit for Human CD4+CD25+ Regulatory T Cells is a simple two-step method that is designed to isolate CD4+CD25+ cells from whole blood or buffy coat. CD4+ T cells are enriched from whole blood or buffy coat by negative selection using RosetteSep® CD4+ T Cell Enrichment Cocktail. The RosetteSep® cocktail contains a combination of antibodies that is directed against non-CD4+ T cells and glycophorin A red blood cells (RBCs). When centrifuged over a buoyant density medium such as RosetteSep® DM-L (Catalog #15705) or Ficoll-Paque™ PLUS (Catalog #07957), the unwanted cells pellet along with the RBCs. The purified cells are present as a highly enriched population at the interface between the plasma and the buoyant density medium. The enriched CD4+ T cells are then selected for CD25+ cells by positive selection using EasySep® Human CD25 Positive Selection Kit. Cells are targeted with Tetrameric Antibody Complexes recognizing CD25 and dextran-coated magnetic particles. Labeled cells are separated using an EasySep® magnet without the use of columns. Cells of interest remain in the tube while unwanted cells are poured off.

EasySep™ RosetteSep™ Complete Kit for CD4+CD25+ T Cells 推薦使用
The isolation of human CD4+CD25+ T cells from whole blood or buffy coat by positive selection

EasySep™ RosetteSep™ Complete Kit for CD4+CD25+ T Cells 產品組成
• RosetteSep™ Human CD4+ T Cell Enrichment Cocktail, 5 x 2 mL
• EasySep™ Human CD25 Positive Selection Cocktail, 1 mL
• EasySep™ Magnetic Particles, 1 mL

 EasySep™ RosetteSep™ Complete Kit for CD4+CD25+ T Cells 保存方法
EASYSEP® HUMAN CD25 POSITIVE SELECTION COCKTAIL AND
EASYSEP® MAGNETIC NANOPARTICLES
Product stable at 2 - 8°C until expiry date as indicated on label. Contents have been sterility tested. Do not freeze this product. This product may be shipped at room temperature (15 – 25°C), and should be refrigerated upon receipt.

EasySep™ RosetteSep™ Complete Kit for CD4+CD25+ T Cells 訂購信息
Stemcell  15862  Complete Kit for Human CD4+CD25+ T Cells  Immunomagnetic Positive Selection Kit Stemcell  15022  RosetteSep™ Human CD4+ T Cell Enrichment Cocktail  Immunodensity Negative Selection Cocktail 
Stemcell  18231  EasySep™ Human CD25 Positive Selection Kit  Immunomagnetic Positive Selection Kit Stemcell  10512  CD25/IL-2R-alpha Antibody, Clone 2A3, PE-Conjugated  Mouse Monoclonal Antibody to Human CD25 (IL-R2α) - PE Conjugated 
Stemcell  07801  Lymphoprep™  Density Gradient Medium for the Isolation of Mononuclear Cells 
Stemcell  15705  RosetteSep™ DM-L Density Medium  Density Gradient Medium 
Stemcell  18000  EasySep™ Magnet  Immunomagnetic Column-Free Magnet 
Stemcell  18001  The Big Easy EasySep™ Magnet  Immunomagnetic Column-Free Magnet 
Stemcell  20000  RoboSep™  RoboSep™ - The Fully Automated Cell Separator    
For research use only. Not intended for human or animal diagnostic or therapeutic uses.